Nerdy Pollen Storage

Hope everyone found Brook Garcia’s pollination tutorial helpful! I sure did!  Again, everyone has their own methods and variations on how to pollinate. My suggestion is — do whatever works best for you! Don’t be afraid to try new things.  Anyway, so in 2009 when I wanted to store the pollen to create crosses with plants that don’t bloom at the same time, I figured I could store pollen in little containers that were available at a local craft store.  I didn’t really want to keep the pollen on a q-tip as I wanted to be able to spread that pollen around to more plants. Saving the pollen in the container would allow me a little bit more control. So being the uber plant nerd I am — I stored the pollen.  Actually, it was fellow grower and friend Jacob Farin who called me a Sarracenia nerd when he saw what I was doing. (OK, yes, I am a Sarracenia nerd. I admit. And I’m PROUD!! YEAH!!)

Yes, that is correct. I stored the pollen.

In little pollen containers.

In a pollen case.

In the pollen storage section of my fridge.

Next to the bacon. You MUST store it next to bacon. If you didn’t know, pollen storage next to bacon actually keeps it viable longer.

(Actually — the fridge keeps the pollen viable longer, not the bacon. But yes, eat bacon. It is good for you.)

You can’t really see it in the photo, but I did tag those cases so I knew what pollen was being used. Good record keeping is key, and organization is a good practice.

When it came time for the pod parent to open up and bloom –  I just simply took my uber-nerdy pollen case and my even more super uber-nerdy pollen brushes and pollinated away!   Here’s a photo from April 2009 of my “Sarracenia Pollen Palette”.

Sarracenia nerdy indeed![A Sarracenia Pollen Palette. It’s nerdy. But it works.]

How to Pollinate a Sarracenia Flower

The following tutorial was written by Brooks Garcia. It is a great reference for pollinating Sarracenia flowers. This guide was what I first ran across (posted on Terraforums) a few years ago when I first started to grow Sarracenia and wanted to get into hybridizing.  I know that everyone has their own preferences, variation, and methods for pollinating, but I found the below to be a clear and concise guide that helped my Sarracenia addiction really bloom. Thanks Brooks!

***

How to Pollinate a Sarracenia Flower
By Brooks Garcia

This is a rather simple procedure but certain precautions need to be taken to insure that: 1) You are successful and 2) You avoid possible contamination from unwanted pollen. Timing is the key here especially if you want to cross species that typically do not bloom at the same time ( i.e. flava x psittacina). With practice and keen observation you will be able to gauge when a flower needs to be protected and when to transfer the pollen.

Necessary Equipment

* Light weight fabric or tulle/bridal veil netting
* Light weight string
* Thin stakes (if needed)
* Q-tips
* Masking tape
* Sharpie permanent marker
* Scissors

Tools You Need
SuppliesNeeded


Flower Structure

We need to discuss the flower structure before hand so you can be familiar with the flower parts and how they function.

Sarracenia in Flower
Photo1

Sarracenias are designed to prevent self pollination by their very structure. The petals are the colorful parts that attract bees and protect the pollen inside the flower from rain and wind. *The anthers produce the pollen and are in the “roof” of the flower and drop pollen onto the style. The style is the upside-down umbrella that has the 5 stigma (female receptors) and catches the pollen. *The stigmas are the ‘hooks’ or slight protrusions on the points of the style which are outside the flower away from the pollen. A bee lands on the sepals (stiff, petal-like structures), crawls down the petals and enters the flower by crossing over the points on the style between the petals thus getting pollen from a previously visited flower on the stigma. The bee gathers the nectar in the flower getting this flower’s pollen on its body and exits the flower through the “dip” in the style and under the petal thus avoiding getting pollen on the stigmas. Look at a flower closely or pull one apart to understand these dynamics.

Flower Parts
800px-Sarracenia_flower_notitles.svg copy
(image courtesy of  http://commons.wikimedia.org/wiki/File:Sarracenia_flower_notitles.svg, author: Noah Elhardt)

Step One: Protecting the Flower

You will need to visit a fabric store and buy a tight-knit, light-weight fabric or tulle/ bridal veil netting. Light weight is very important because you don’t want your flower stalk bending under the weight of wet material after a rain. Staking the flower stalk is an option as well on weak-stemmed species like rubras. The netting will only be needed until the pollen has been set and until shortly after the petals have fallen off. Then the netting can be removed.  This is where keen observation is handy but err on the side of caution and “bag” your flower before the petals have fully opened.  How will you know?  The petals will still be cupped close to the style.  The photo below shows the flower fully open and at this point on this particular flower, the pollen has begun to drop onto the style. It should have been bagged sooner.

Flower Fully Open

The style is the five lobed structure that is the bottom of the flower. This contains the female parts of the flower, 5 stigma in all. On the smaller species (rubras and psittacinas), the flowers are going to be quite small and two people maybe needed to carry this out; a small artist’s paint brush maybe a better tool than a Q-tip for gathering and transferring pollen. Cut a large enough square of netting to cover the flower loosely and be tied with the string. I start at the back of the flower and tuck the fabric between the stem and the back of the sepals. The sepals are the stiff parts that are left after the petals drop. Then wrap the flower loosely and tie it off until you are ready to pollinate the flower.

Bagging Procedure

After each pollination session, secure the netting until the petals have dropped and the flower tilts upward.

Step Two: Collecting Pollen and Cross Pollination

You will need to check the flower every day to see if the pollen has developed and begun to drop onto the style from the anthers. It will look like small pale yellow particles. Pollen will drop for several days up to a week and can be gathered off the anthers if necessary. The anthers, the male parts, are in the top of the flower inside under the petals. Untie the netting and carefully unwrap the flower. Take a Q-tip and very carefully lift a petal.

Lifting Petal to Expose Flower Parts

Through the “dip” in the style, insert the Q-tip and roll it between your fingers to gather the pollen on the cotton. Withdraw it carefully. Take the Q-tip with the pollen to the flower you wish to cross (you have unwrapped this flower as well) hold the stem of the flower and touch the Q-tip to the stigma. *Remember this is protruding between the petals, and roll the Q-tip up the stigma lightly.

Transferring Pollen

Be sure to do all 5 stigmas. Each stigma has a tube in which the pollen will grow down to reach the unfertilized embryonic seeds in the ovary. Each chamber in the ovary is capable of holding 100 seeds. Re-bag each flower and repeat the process the next day for three of four times to insure fertilization takes. A sure sign you have been successful is that the petals drop and the whole flower tilts up and the ovary swells.

Successfully Fertilized Flower Pod

If you want to pollinate a plant that is not in bloom yet, pollen can be saved in the refrigerator on a Q-tip in a plastic bag or wrapped in foil for several weeks until the flower comes into bloom. To “self” a plant, simply take the pollen and apply it to the stigmas. Reportedly, Sarracenias do not “self” very well for some reason but with multiple applications of pollen you should get good seed set. I label the cross by simply wrapping a piece of masking tape on the flower stem with the name of the pollen donor (father). This may have to be replaced once before the seed ripens in the fall. When labeling the plant produced, the pollen receiver is shown first. It is not necessary to wait until the seed capsule breaks open or turns brown to collect the seeds. Seeds are usually fully ripe by mid September.

Now, ain’t plant sex grand!? Good growing!

A golfball(ensis) with Judith Hindle influence…

The following is a seed grown clone of Sarracenia psittacina “golfballensis” (sorry that link is only to a list of photos to Sarracenia psittacina’s, at least you get an idea; and for some reason I can’t find any photos of the variation “golfballensis”) and Sarracenia ‘Judith Hindle’.

This cross was created by Brooks Garcia, and the seedling below is about 2 years old. S. psittacina “golfballensis” gets to have large sized hoods/heads, the size of a golf ball (hence the name).  Below is pictured only one of several seedlings that I am currently growing, all are a bit different, but this so far has been the strongest seedling of the batch.  It’s only 2 years old but is putting on some decent size for it’s age, and showing good color. I am looking forward to what it can do as it matures.

Sarracenia psittacina "golfballensis" x 'Judith Hindle'[Sarracenia psittacina “golfballensis” x ‘Judith Hindle’ 2 year old seedling – cross by Brooks Garcia]

Sarracenia "Bug Bat" x "Diane Whittaker"

One of my first planned hybrids was created a couple years ago. When I was really started to get into this thing, a couple of friends of mine were kind enough to really ignite the Sarracenia obsession in me.  I received a division of Sarracenia “Bug Bat” from Wes Buckner, and a Sarracenia “Diane Whittaker” from Brooks Garcia. (Thanks guys!!) So a couple of years ago, they both bloomed for me — and you already know what I did with that… 😉

Photos below are of 3 different seedlings. Each has similarities and differences, but you can see both parent plants in the mix. 😉

This is in honor of two of the guys that got me addicted…
Sarracenia "Bug Bat" x "Diane Whittaker"
[Photo: Sarracenia “Bug Bat” x “Diane Whittaker”]

Sarracenia "Bug Bat" x "Diane Whittaker"
[Photo: Sarracenia “Bug Bat” x “Diane Whittaker”]

Sarracenia "Bug Bat" x "Diane Whittaker"
[Photo: Sarracenia “Bug Bat” x “Diane Whittaker”]

Sarracenia courtii x 'Adrian Slack'

This particular hybrid is only a few months old, but is showing  some of promise. It’s one of the few S. ‘Adrian Slack’ hybrids I did last year. The pitcher is still too young to really tell what the mature pitcher will look like,  but a few hints and notes of what it may be like could be can be seen.  Another interesting plant to look forward to in the future. 😉

Sarracenia courtii x 'Adrian Slack'[Photo: Sarracenia courtii x ‘Adrian Slack’, under 1 year old]

Come to the Light

One of the many things I find so captivating (literally) and fascinating about carnivorous plants and Sarracenia are all of the many systems in place that lure in and capture their prey. Deadly beauty in both art and function. It’s all friggin’ awesome stuff!

The below photo is one that I managed to capture of a freshly opened Sarracenia excellens as the morning sunlight was filtering through this plants areoles. The hood will eventually fold over that opening. As the insect is attracted to the tube by the scent and nectar the plant will produce, it gets lured in deeper into the trap. Eventually, the meal wanders too far in and then tries to escape. Said meal sees the “windows” and thinks it can get out that way. The windows provide that “false hope” of escape for those bugs unfortunate enough to wander too far into the hole. As the meal tries to escape, the futile efforts lead to exhaustion and ultimately a nice meal for the plant.

Yes. Come to the light…

Sarracenia leucophylla x minor var. okefenokeensis

[Photo: Sarracenia leucophylla x minor var. okefenokeensis]

Stuffing Envelopes…

Yeah. Tonight I was stuffing envelopes… Literally. I get enough paper-pushing at my desk during the day and then I get home and this is what I was just doing for the past 2 hours…

filing1[Paperwork! AHH!]

But I didn’t mind too much tonight cuz I was taking this…

pod1

[Photo – Seed Pod – Sarracenia (rubra ssp. jonseii AF x minor var. okeefenokensis) x “Green Monster”]

Stuffing envelopes like this:

packet1
[Stuffing Envelopes!]

And getting something like this:

filesystem1[The Sarracenia Files]

And that’s not even all of my crosses on the year so far – still have a few to go through that aren’t quite ready.  Oh well.  This is the “paperwork” I’d rather be doing though! Love it!!